Lab Protocols

Double digest RADseq protocol - Standard PCR

This protocol is a simplified version of Peterson et al (2012) PLoS One written by Steve Doyle and myself.

Ensure your library will have sufficient diversity before starting. A useful barcode and index diversity calculator can be downloaded from https://nematodegenetics.wordpress.com/protocols/

Download: Protocol

Double digest RADseq protocol - qPCR

My updated version of the above protocol using qPCR. This protocol uses real-time PCR to add Illumina indexes. RFU values obtained from RT-PCR are then used for pooling, saving time and money on quantification prior to pooling.

Ensure your library will have sufficient diversity before starting. A useful barcode and index diversity calculator can be downloaded from https://nematodegenetics.wordpress.com/protocols/

Download: Protocol